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The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using
Because of the overwhelming plethora of studies, this book, written by leaders in the field, arrives as a welcome road map for beginners and experts alike. The volume provides a broad overview of the basic mechanisms and applications of RNAi to different model organisms for functional gene analysis, including
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The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way.
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RNAi: A Guide to Gene Silencing. Edited by Gregory J Hannon. Cold Spring Harbor (New York): Cold Spring Harbor Laboratory Press. $130.00. vii + 436 p; ill.; index. ISBN: 0–87969–641–9 (hc); 0–87969–704–0 (pb). 2003.
The final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. This book is a comprehensive guide to gene silencing by RNA interference methods for beginners and experienced professionals in the
The RNA World has expanded significantly with the discovery of a new genre of small RNA molecules, the small silencing RNAs (siRNAs) and the microRNAs (miRNAs). In the wake of the wave have sprouted kits and companies—designed to do everything from knock down expression of your favorite gene to cure hepatitis.
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Jul 1, 2013 RNAi: a guide to gene silencing. Hannon, G. J., ed. (2003) RNAi: a guide to gene silencing. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.. ISBN 978-087969704-4. URL: wwwcshlpress.com/default.tpl?action=full&c.
     

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